Conidia are formed from conidiophores, which are specialized asexual structures. nidulans primarily reproduces asexually via the production of conidia 6. nidulans produces sexual fruiting bodies called cleistothecia. nidulans undergoes two reproductive modes: sexual and asexual. nidulans, several other Aspergillus species have been used to study biological processes in filamentous fungi 5.Ī. Aspergillus nidulans is an important model organism for filamentous fungi that is essential for genetic and fungal biology research but also produces a mycotoxin called sterigmatocystin 4. Some Aspergillus species can be used for food fermentation, enzyme production, and research purposes 2 however, others act as opportunistic human pathogens or mycotoxin-producing fungi 3. nidulans.Īspergillus species are filamentous fungi that are commonly found in soil, seeds, grains, and foods 1. Overall, these results suggest that CsgA is crucial for proper fungal development, spore viability, conidial germination, and sterigmatocystin production in A. Additionally, the production of sterigmatocystin increased in the Δ csgA conidia compared to that in the controls. Further analysis revealed that the Δ csgA strain exhibited delayed conidial germination and abnormal germ tube length. Transcriptomic analysis revealed that the loss of csgA affects the expression of genes related to conidia germination, DNA repair, and secondary metabolite biosynthesis. In conidia, the deletion of csgA resulted in increased trehalose content, higher spore viability, and increased tolerance to thermal and oxidative stresses. Overexpression of csgA led to decreased conidia production but increased cleistothecia production, suggesting that CsgA is essential for proper asexual and sexual development in A. The deletion of csgA resulted in impaired production of sexual fruiting bodies (cleistothecia) and lower mutA expression levels.
nidulans, the Δ csgA strain produced abnormal conidiophores and exhibited increased conidial production.
In this study, we identified CsgA, a conidia-specific Zn 2Cys 6 transcription factor containing the GAL4-like zinc-finger domain, and characterized the roles of CsgA in the model organism Aspergillus nidulans. mainly reproduce asexually via asexual spores called conidia.
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